Research Question: How will the D.O, Turbidity, temperature, and Depth of three separate locations on Seneca lake affect the plankton population of those three locations.
Independent Variable: Depths of the three locations.
Controlled Variable: The testing of the other variables will always be done on Seneca lake.
Other Variables: The Dissolved oxygen levels, Turbidity levels, Temperature, and Plankton population.
Abstract: There are many things that influence the population of Plankton in any given marine environment. “Ecological Regulation” a scientific article written about the limiting factors of plankton suggests temperature, sunlight intensity, and nutrient availability as the chief limiting factors for any given plankton population. The Conclusion was supported by “Phytoplankton diversity in response to abiotic factors along Orissa coast, Bay of Bengal” by Swati S. Panda, Dhal N.K, Panda C.R who over the course of a few years collected data on plankton populations, and on the environments they lived in. This data supports “Ecological Regulations” conclusions about Limiting factors of Plankton populations, and has numerous pieces of field evidence that support this same information. Another important aspect to “Phytoplankton diversity in response to abiotic factors along Orissa coast, Bay of Bengal” was the data gained on the type of Plankton that were alive, and what they ate, by studying this they were able to conclude which nutrients were most abundant in the area, and which species of plankton were most resilient to change. The final limiting factor of Plankton is which season the area involved is currently in, although it is not the season that causes the change as much as it is the temperature. Throught data referenced in both the “Ecological Regulation”, and “As The Seasons Change, Will The Plankton” plankton populations will typically be highest in the summer months, begin to fall in the fall months, but will be higher than the populations seen in spring (this is data on a location that has 4 seasons instead of 2). Through this research the question of how temperature affects plankton is answered, however it isn’t as simple as saying plankton prefer high temperature. The information in the third article suggests that some plankton will respond negatively to the rising temperature of water (global warming wise), while a few may then thrive due to adaptability, and less competition. The Conclusion the final article made was while we can make short predictions about how temperature affects plankton in an individual area, we simply lack enough knowledge on plankton, and global warming implications to make appropriate predictions.
Hypothesis: I hypothesise that the areas with high Plankton populations will be the areas of the lake that are the warmest, have the highest Dissolved Oxygen, the clearest waters, and the areas of water that are closest to the surface. I believe this because the evidence i gathered in the abstract portion of this assignment stated that the limiting factors of Plankton population were Intensity of Sunlight, Temperature, and Nutrients. So it is reasonable to conclude that the area that receives the most intense sunlight (lowest Depth), has the highest temperature will have the higher levels of plankton. Finally the Dissolved oxygen affects the population because as all these organism use oxygen in their respiration process, so the more they have the more easier it will be for them to survive and reproduce.
Method: In all three seperate depth locations i will mimic the process by which i collect temperature, Dissolved Oxygen, and turbidity (shown in the procedure stage shown below. By mimicking all these processes i will help to eliminate human error, and collect more reliable results. Along this same principle of thought i will collect the Plankton sample in the same manner at all three locations to once again help limit human error.
Procedure:
D.O Collection Steps
- When you get to the lab bench, gather the dissolved oxygen kit.
- To the LaMotte sample bottle, add 8 drops of the manganese(II) sulfate solution (bottle 4167) followed by 8 drops of the alkaline potassium iodide azide solution (bottle 7166).Some water may drip off the sides.
- Carefully cap the bottle, mix by gently inverting (, then allow the orange-brown precipitate that has formed to settle below the shoulder of the bottle (about 3-4 minutes).
- Using the 1 gram spoon provided in the kit (0697), add one level spoonful of sulfamic acid (bottle 6286) to the solution in your LaMotte sample bottle. Cap the bottle and mix until both the reagent (white crystals) and precipitate (brown crystals) have completely dissolved and you obtain a clear brown-yellow solution.
- Pour this clear brown-yellow solution from the LaMotte bottle into the titration tube and fill it up to the 20 ml line.
- Use the plastic eye-dropper provided in the kit, add 8 drops of the starch solution to the titration tube. At this point, the solution should change color to a bluish-green.
- Fill the Direct Reading Titrator (0337) up to the 0 mark [looks like a syringe, marked 0-10 ppm] with the sodium thiosulfate solution (bottle 4169).
- Insert the titrator you just filled through the small hole in the cap of the titration tube and titrate the solution slowly. Swirl the titration tube until the blue color of the solution disappears permanently with one drop of titrant (i.e., you are looking for a color progression from green-blue to blue to light blue to colorless). You may have to fill the titrator more than once. Be sure to record how much titrant you used before refilling. The direct reading titrator is calibrated in units of parts per million (ppm) dissolved oxygen, therefore, be sure to record all of these units.
Position and Depth
- When Ship has made complete stop mark the exact position of the ship, by either using the ship's radar or by using the G.P.S.
- When Ship has made complete stop mark the exact depth of the ship using the depth finder on board the ship.
Temperature
- Use the Ship's onboard "CTD" to gather temperature measurements.
Turbidity
- Take Cup that has Secchi Disk on it and slowly lower it into the lake until it disappears. Record the height at which it disappeared.
- Pull the Secchi Disk back up until it can be seen again record that.
- Repeat steps 1 and 2 3 times, and collect average overall.
Plankton Collection
- Twist the end of the rope around one hand 2-3 times and grasp with a fist. Don't let go! This grip is to ensure the net isn't tossed overboard when it is cast.
- Make sure the clasp at the bottom of the net is closed! If it isn't, the sample will not be captured and the net will need to be recast.
- Lower the net over the side of the boat until it floats freely in the water. Walk slowly
from the stern to the bow of the boat and then back again, gently dragging the
net behind you. Try to walk at a steady pace so that the net stays at a fairly
constant depth and does not scrape the side of the boat. Since water clarity is an
indication of the presence of phytoplankton, use your secchi disk reading as an
indicator of productivity. If the secchi disk reading is less than 7 meters, traverse the
length of the boat twice. If it is greater than 7 meters, make 3-4 trips to make sure
you collect enough plankton in your net.
- Back at the stern of the boat, gather the line up until the net is vertical, hanging freely, and level with the railing. Using the provided wash bottle (filled with tap or lake surface water, not distilled water), wash down any plankton clinging to the sides of the net into the small grey cup attached to the lower end of the net.
- Raise the net slightly, keeping it vertical. Grasp the grey sample cup and swing it on board, making sure not to spill the sample
- .Hold the provided plastic beaker under the sample cup and attached rubber tubing and release the tubing clamp, allowing the sample to flow into the beaker. If it appears that some sample has clung to the inside of the grey sample cup, carefully use a small amount of water from the wash bottle to rinse it into the beaker. You don't want to dilute the sample.
- The beaker can now be taken to the lab for analysis. Remember to rinse it out
when the plankton sample is no longer needed (using either tap or distilled water)
and replace it in the net box.
Final Question: What is the average overall productivity of Lake Seneca, and has this productivity on average trended up, down, or remained constant.
